TNFRSF1B Knockout cell line （Hela）

TNFRSF1B Knockout cell line （Hela） Order

Catalog#

YKO-H920

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TNFRSF1B Knockout cell line (Hela)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Gene Name

TNFRSF1B

Gene ID

7133

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 3 (based on transcript TNFRSF1B-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hela			STR Info (Cell bank) Cell Line:HELA
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	13.3		12	13.3
D7S820	8	12		8	12
D16S539	9	10		9	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	9	10		9	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TNFRSF1B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hela cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TNFRSF1B gene knockout TNFRSF1B Knockout cell line (Hela) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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